CR70517

CRIMIC CR70517

Gene U3-55K

CG33505

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:14858845..14860758	51C5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTGTTTTTTCGATTTTCAATTTACTTGG	ACCATGACTAGCTATTGAAAGTTGCC

Images

Construct Design Sequences

sgRNA sequence	GTGGAAAGTAACTAGTTGCGCGG
sgRNA2 sequence	CATTTGTATCTTCTACCTGAAGG

left_200bp	right_200bp
GCCTTGCGTATAATTTTTGCGCCAATTTTAGGGTGGCCGTGTTGACTCGCAGTTTCATAGCATTCAGCCTGTTGATGTGCACTCGCCAAACAGCTGATACCACAAAAGTACTAAATTTGGACACGCGCCTTATATGCACGTGGAGACGTTCCTTCGTTTAAATTAAGTTTTTTATCAAAAATATTGAATTAAAGCCGCGC	TGAAGGTTCTTACTTTTAAACATATAAAATTTATTTGTTCTTTATAAACAAGATCAAACGAATGTTTTTGAATCTATTAAATTTGCCTCATATTGAAATTTAACTCCTTATATTAAAGTAAGGGGTATCTGGTCGTCTATTTCCATTAGCTCAAATATTTTTTCTAAAACCGATATTCGTTTTGTTATAACCGCAAATGG