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CRIMIC CR70518

Gene Ssl

CG13591

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:24499243..24500267	60D1	FBti0226943	97339

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCAACTGGAGCATCAGG	GCACTTGATGCCAATTCTCGAG

Images

Construct Design Sequences

sgRNA sequence	GTCTGTCATTATGAAATAGTAGG
sgRNA2 sequence	ACCCATGTGTTAGTGGTCGCTGG

 

left_200bp	right_200bp
CGTTCTGGTCATTACAGACGTGTTTTGCGGCCTAGGCTTCCAAGAAAGCCAGGCTTCTGGCTAGCCTTGCCGATTTGACGAGTTTGAGGTCTTTGTGAAGGGCATCGAGTCCTCGGGCGATAAAGGGTTTGGAACACTGCTGCCCTGTCGCAGCCCTGACATTTGAATTGTTGTCACTGAGCTGTCTGTCATTATGAAAT	ACCACTAACACATGGGTGCACTCTCTCTGCCCTAAACACTTCACACCATTCGGTCCACGCTGGCCATATCAAGCAGTCATTCGAAAAGCAAAGCGTACTTGCCGTTAATAGTGGTAATCACCTGGGAGTTCGGAAGTGAAGCAGCTTTAAGCGGCCTATTCATCCGCTTAGTGTTGTCAGACACTGCGAGAAAATGGTTG

 

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