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CRIMIC CR70519

Gene Upf1

CG1559

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:11899996..11905371	10F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	CGAAACGCACACACGACAC	GCGCACTCGTGACCAATG

Images

Construct Design Sequences

sgRNA sequence	CTGTTGTTTCTTCTCACCCAAGG
sgRNA2 sequence	CAATGCGACCTAAGAACATTAGG

 

left_200bp	right_200bp
GCCATACCATAATCATCGCGGCCACGCTCCAAGCTTAGGAATTTTTCAATTCCGGAAACACTGACCAAGCGATCCGTGGTCTAGGAGCAGGTACTATAGGTGAGCATCAGTCCCATCCGAGTTACGTACCTATCGGCCAATGCACATTGCTAACTAGATCTCATCGCGATCCCTGCGGAAACGCAGCATAATCACCTTGG	ATTAGGACAAAGCGGGACATTAAAAGATGGCAAATCGAGACGAGGCAGCGTGATAGATGGAGACGGATATGGCAAGATGTGGAGTTACAAAGTAATAAAGAAGACTGAGACGGGAGAGAGATGTCGAAAGATCGAAATATCGAGAGCAAATCACTGTAGGCTGCCAGGATGCACAGCTGCAGTTGCTGTTTCCGTAGCGC

 

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