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CRIMIC CR70521

Gene CG5913

CG5913

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:25893495..25895026	96F6	FBti0226945	97341

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTTCTTTCATTTGGGCTTTGGG	CATTAGACTTAGTCCGTTGCCCTC

Images

Construct Design Sequences

sgRNA sequence	CACATTGCCACGCCGGTGAGAGG
sgRNA2 sequence	ACAAGAGGATCAGTCGTTTATGG

 

left_200bp	right_200bp
ATTTTAATTACTTTTACCAAGTCTTTATATTATATATAAATTCGTTTAAAATAAGTTAACGAAAAATGTTTTTCAAAATATTTGCATGGTATGTGCCCGTGCTAACCTCGCAGCGCTATTTTGACAAGTTGGCAGGTTGGCTTTGGAAAAGTTTTTGATATTTCCGACCCACAAAGCAATGGTCACATTGCCACGCCGGT	ACGACTGATCCTCTTGTAAACTACTAACATTACATGTCTTTAATCGCACGCATACCCCAGTTTAATTTATTGCGAAATTTAACGAATTTGTTAATTATTGCAAATGGGCAGGTATTTATAAAATAAACTAAATATGCCATCTATTTGCCAGCAAATCTCGCGTGTCGATTTCGCTTTTTGGGAATCTGGGTTAGGTGTTT

 

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