CR70522

CRIMIC CR70522

Gene CG8237

CG8237

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:8925671..8926890	44F8	FBti0226946	97342

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCATTAGATAAACAGCTTCTTGTTCGG	GAACTTCATTATCATTCAGTTTCTAGTACCAGTG

Images

Construct Design Sequences

sgRNA sequence	CATTGTGAGACTGAGAAAATGGG
sgRNA2 sequence	TGCGCCATTCCTACACACTTTGG

left_200bp	right_200bp
TCAAATATTTCGCATCAAAAGTTAAAGTGAGTCTTCTTAAATTTTCCCGCCCTGCTTGAAATTCACAGGGAATCGTATTTAGCAGTATTTGTCATTCGCAGCGATACGCCGACACACCCTACCGATTGCAATACACGTCAACTTGTATAAATAAATAAAAGCCAGCCAAATAACAAAATCGATCATTGTGAGACTGAGAA	TGTGTAGGAATGGCGCACTCCTATGCACTTCCTATGCACATATAAATAGACTTGTTAATGATAAATTTAATGTCGTTATGTACTGCTGTCGTGTGAAATAAAATAAAATATCACATGAATACCTCAGAATCTGCACGATAATCAAACATAACCAGTAATCCCAATGATCAGTAGAGCTTCCGATATTCCTGTAGAGCTAC