CR70523

CRIMIC CR70523

Gene CG12320

CG12320

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:18251069..18252734	90F10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CCACTTACACAATAAAGTGAAGAACAGC	CATGCCATACTCCTTGCAGTCC

Images

Construct Design Sequences

sgRNA sequence	ATTGTTCGCTTGCAATTATTAGG
sgRNA2 sequence	ACTTCCGGAAGCCTAGGAGAAGG

left_200bp	right_200bp
AACAAAGTCTAAATACAATGTAATTATATCAAACAAAAGATTTACTTAAAAAAAAGTATATATAAATTGAATTTGCCGCTTGAAAGAATTGTTTGTCTAGTGTTGCCAGACTGCTCACATGAAAGAACTTATCGAAATAGCCGTCACATCTGGCAACTTGCTGAAAGGCCCGCACTTGCAATTGTTTTCTTTTTCCTAAT	AGAAGGGCGCTACGCATGTGAAGTGTAATTCATATATTTGTAATACAATGGACTGAAACTGATCTATATTGATTTCTACTTTAACGCAGCGAATGCTGGGTGCGCAAGTAATTCCACAGATCCCGAGCCGTGCGGATGCTGACCATGAATTGGCACAACTTGGTCTCGATCAGGCAGAGAATCAAGAACTTGGCCCGCTT