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CRIMIC CR70525

Gene DNA-polA50

CG7108

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_SA_kG4	3L:8340314..8344404	66C7-66C8	FBti0226966	97343

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGATTCAATTTGTTTCGACCC	GACGATAAGGACGGCAACG

Images

Construct Design Sequences

sgRNA sequence	GGATATAATCATAAGATATGTGG
sgRNA2 sequence	ACTGCAAGCTGGCAGATTTCTGG

 

left_200bp	right_200bp
ATTAATAAGCCTCTTACGTTTAAAAAATGCATGTCGTTAATATATTTCAGTACTTTAAGCTCCAATCGTGTATCTCTCTTTGAAAAAGAAAAAATCAGCAAAAAAATATATAAATATAAGGTTTCGACAAAAAGCTGAATGGTTTTGATTTCTTTACGGTTATAAGTATTCTGTTCTTTTTCTGGATATAATCATAAGAT	ATCTGCCAGCTTGCAGTTTTGATTCTAATTTTACAAACTAACATAAGAAAATAAAAGGTTAATGAATATTTCAGTATGCATACACTTTCTCGTTTGCCGCTTGCGTATTTATCTTTATTAAGTTTTTGTATTGTAATCATTAGGCTAGCAACAATAAATAGTCTAGGCGACATCGCACCGGTTCCGGTTCACAATCCGGC

 

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