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CRIMIC CR70526

Gene gpp

CG42803

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:6406855..6449274	83E7	FBti0226893	97194

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCCAATCAATCAATCGCTGTC	ACGTGTGGTGCGAACAGTTC

Images

Construct Design Sequences

sgRNA sequence	TAAAAGAGGCCCCTCCTATACGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATAGAAAATGTATCAAATCAAATTCTTTTGTATTTATCATATCATATCAATGAAATCATCAATTGAAGTTTGGGATGATAAATACGTTTAAGTAATGATAACCTGAAAAGGAATATAACTGAATATGTGTATATTTAGTATTAGACTTTGTGGATTGTTAACGATTTAAAATGTGAAATCGTAAAGAGGATTGTCCGTAT	AGGAGGGGCCTCTTTTATATTTGCTCCGTCTATTTAATCAAATCAAATCATTTACCTATATTTACAATATTCAATGGATTTCTCAAAAATGAGTTAAAAAAAGGCACTAAATCCATTCTAACGAGATAGAATACTAAAATTAATTAAGGGCTATAGGATAGGTGCACACAAGAGAGCATTCTTATTTGAAATAGATATTA

 

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