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CRIMIC CR70527

Gene Blos3

CG34255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:18870224..18870897	75E2	FBti0226947	97344

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGATTACATCGTCTTCTGAGTAATGGG	CACTATACGTCTAAGTCAGGTTGC

Images

Construct Design Sequences

sgRNA sequence	GTATTTAATAAACTGAATGATGG
sgRNA2 sequence	TCGAAAACAACATTGCCATTTGG

 

left_200bp	right_200bp
TTGTTACTAATACCATTCATTATCCGTTAATAATTGTTATGATTGTAGTTCTACGGATCTGTTAGCATAGATATCACAAACATAAGAATATCCCCTTCAACTGCGCCCATGTGAGCAACAGTTTGCAGTTTTAACTGATCTGGCAGCGCCCTAACTGCAGTAAATAAAAAATTGTTTACAATAGTATTTAATAAACTGAA	ATTTGGAGATCCCTTACCGCTTTCACTCGCAGCTTTGTGTTGAGTGCATCAAAGCAGCTCGTAACCACTGATCAAATGCTCATCAAGTCGCAACATAGTCTTCAGTCGGCTCACACATCCCTGCAGCAGGCCCAGAAGAACGCCCAGGACCTGCAAAACAGGGTGGCTGATGTTATTACCAGCAACTTCCTCCCGCACAT

 

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