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CRIMIC CR70529

Gene gek

CG4012

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:24092079..24098636	60B9-60B10	FBti0226894	97195

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGGAGAATGCGGAAAATATGGG	CGTAGAATTTGGTGCGTGAGTC

Images

Construct Design Sequences

sgRNA sequence	CAGTGGGTGCGGCATTGTTGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATATAGCGGAATCCATTAGGAGTACGGCCTTCTGCTTGCGCCCATTGTTCTCCGGTCTACCTTCCCCCACGATTCATCTTGGTTTTCCATAGCCATAGCCACAGCCATAACCCACCCGTTTGAGCTGATTTCTGCCCCCCGAGCCGCATTTCTGAATTGGAAGGAGCCAGGTGTCTGCGACGTCAGTGGGTGCGGCATTG	TTGCGGGCTGGCAAATGTTTGCGATGCATGTGTTGGTCGGCTTTTTGGGGTAATACAACAATTGCTCGCGCTCTTGGCCAAAGGAGGACAGTCTCCGAGTTTTTATCAGCTTACATACATATGTGTTTTTTAATGATGCCAAGGATACTTACGTATTAGGTTTCCACAGCACTTTAAATATCTAAGCGTTGAGTTAAAAA

 

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