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CRIMIC CR70532

Gene SA-2

CG13916

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:1434336..1437872	62A1	FBti0226950	97347

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCCTGTTTTTAATTTATTCGTGTCCC	GCTTTGCCAAATGCTGCCG

Images

Construct Design Sequences

sgRNA sequence	AGTTGTGGTTCATTGGTTAATGG
sgRNA2 sequence	TGTATCCTTCAAGTTGCCTGAGG

 

left_200bp	right_200bp
AAAACACGTGTTGTATTGATTTTTAAGAAACTATATCTGAATTTATATCTGAAAAACTGAATTGGCACATTGGTGTATGTATCTTGTGACTTTTCAGTTAGGTTTTATAATTTTTAAGTTTAATTTTTAGAATTGTTTGCAGCCCTGAATATAACCTCAAATTTCAAAAGTTCGCTTTATGTCAGTTGTGGTTCATTGGT	GCAACTTGAAGGATACAACTTACAGGATGTTATATGTATCTATATAATGCTAAACTCGTTATTAAAGATTTTCAGTCTGCATTATTTATTATTGGTTTCGATGGTGTTCACTTCTCATACGTTTGCAATTGCGTGTTCATCCTGTTTGCCATTTTCCGTATGCGTTTGTGTGCAAAAGGAAAACTCAGTTTTCATCTCCA

 

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