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CRIMIC CR70533

Gene Sec24AB

CG1472

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9876232..9882357	46C1-46C2	FBti0226951	97348

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCATTCCATTCGATCACTGC	GACAAGTTGGCTCTTTCGCATTTACTATG

Images

Construct Design Sequences

sgRNA sequence	TCGACATGCTGCTAATTCATAGG
sgRNA2 sequence	ATGCTGCATACAATAGTGGTAGG

 

left_200bp	right_200bp
TGTCTGTAAGCGGGGAAAAGCATCCCGCTTTAAAACTTTACAACTTTTATCAGCCGCACATTGTTTAATGTTACGAGCTTTTTAACATTGAACGATAAGATAATTCCCTGTTTCATGTCGGCAGCAACGCAACCTTCGCTAGTGATAAGATAGCAACTAAACAAAATGTAATTCTTCATCTTTCAGACCACAAACCTATG	ACTATTGTATGCAGCATAAATACGAGTTTCTAAGAACCTAAAAACTCAGACTCTGGTGACTAGAAGCACGAAGCGAATGCAGGTGACACTGGTGCGAAACAATGGAGACCAGTTCGGGACAGACATACACTTGATACACCTTAACCACCCATCCCCTACCCACTCTCTCGGCTAAGGTAATTTTAAAGTGCATGTATTTA

 

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