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CRIMIC CR70534

Gene Rep

CG8432

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:19492087..19494125	56D13	FBti0226952	97349

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGATTTGGGCATTGGGAAATTCAG	AAAGGAGAAACATTTTTATACGCATTGG

Images

Construct Design Sequences

sgRNA sequence	TTTAAATATCAGTGCAAATAAGG
sgRNA2 sequence	GTAATGATATATGTAGACCAAGG

 

left_200bp	right_200bp
AAATAGTTACAAAAGTTTAATAATATTTTAAATTTCAAACAGCTTTATAACGTTTTGTTTCGTTAGCCAATACTATGAACAGCTGTTTGTTGGTGACGTCATCGCTAGCTGCGCATCTGTAAACAGCTGCTTAAGTAAATTCGCAATAAATTCACGGAATACTTTATTTATCTGTAAATATAGTTTAAATATCAGTGCAA	TCTACATATATCATTACTTATTACAAAATTTTCTTAAGTATCTTTCGAATAAGTTATATATTGTATGCAATTTTTTGATTCATATATTCCTATATATTTATTATGTAAATTATTTATAATTGCTAAAAGTACTAGGTATATATAAATGTTTAAATGTGAGTGTTTCTGTGCATTTTGCTTTGATAAATATATGTTCGAAA

 

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