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CRIMIC CR70535

Gene Ance-2

CG16869

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:13909333..139117190	34E2	FBti0226953	97350

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTTCTCACACACAGTTCATTG	CATTATTTGCCTTTTGATTGTGGCATAC

Images

Construct Design Sequences

sgRNA sequence	ACGACAATCCATGAATCCATGGG
sgRNA2 sequence	ACTTTCAACCTCTGCAAGACTGG

 

left_200bp	right_200bp
TTTATGGTTGTGTAAGTTAATGTTCATGATATATGAAATACCCCATCCAAAAACAAAGTGCTGGACATAATTGTCTCAGTAAACAAGTTGTAGATATTGCCAAGTGAAGTCGTAGTTGTATTTTCTAAGTGGAACACATTATAATTACAAATGGCCAACTGGGCGCTCAGTTGGTCACCAGACTCACTCCACTGCCCATG	GACTGGTTGCAGCACCAGAATCAAGAAAACAACCTGGAAGTGGGCTGGACATAAGATTTGGTGCAGGGGATATTTTGGAAATTTAATCACTAGTAAATTATTTAAATAAAAACATAATAATGGAAGGGTATTTAGCACAGTCTTTCATAAGACTTCCACCATAAAGTTGTGTGTGGAAATGAACTCTTTGAATTTAATCA

 

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