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CRIMIC CR70537

Gene CG13920

CG13920

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:1648944..1651501	62A9	FBti0226895	97196

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTGTTTGTAAATTGTTTTGAAAACCTCC	GAGTGTTTGTGGTAATACGGGG

Images

Construct Design Sequences

sgRNA sequence	ATAAACAATAGGGTGGCCTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATATATAGAACAGCTGTTGCTCAGAATTTTTTGTTTACCTCTTGTAAAAATAACGCCCAGAGATTTTCGTTTTCATTACCATAAAAATAATTCGCATACCAAAGTAAACATTTGCGAACACAAGAACCACAATGACGTGTTCTTTTCACTCAATTCAGAGTATACAAATTGGTTTTATATACATAAACAATAGGGTGGC	CTGAGGGATTATTCAAAAAACTTTATAGTTTTAGAGATAAAAGTAAATAAATAACCAACTAGATCATCCAAAAGACACTTCAGTTCAATACTTTCGAAAAAAATCAGTATTCAGATGGCGTTTTCGAATTGGCATTGCATTCGTTACGCGCCATTTCACTTTCAATTTTGAAGATTTCTCAATCTCGTTTTTCCCGAAGG

 

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