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CRIMIC CR70539

Gene Dbp45A

CG12759

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9087834..9089802			98545

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTTACCTGTCTAGAGCGTGAC	ACTGAATCCAGTGATGCGCC

Images

Construct Design Sequences

sgRNA sequence	AACGTGCGCTGTATTAGTGATGG
sgRNA2 sequence	GCTTGGTTGGGGTATCTTCTTGG

 

left_200bp	right_200bp
ATTAATTATAAAATGTATTATTAATTTTATAGTGAATTTTTTCTTATTACTCGCCAATAATTCTGCGGTTGAAGTAAATAAAAAATTTAATTGAATTTAAATTAAAAATGCGTACTAAATACAGCTAACCACTCACACGGAGCAAAAATTAAAAACGTATCGATAGTTATATACCGATACAGAAGACAATACTTCCATCA	AGATACCCCAACCAAGCCACTGAAAAGGCTTAACAAAGAAAAACCAGTTGCCCAAAAGGGCAGAGCAGATGTCAAGAAAGATAAAGCGTGATGTTTAAATATACTTTTATAAAATAACAAAATAACTTTTGAGCTTACACTGCTAAGTAGTTGCAAAATGCTTACTTAAATAATTTCAAAAATTCAGACATGCTAGAGCG

 

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