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CRIMIC CR70540

Gene DNApol_alpha73

CG5923

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:27239163..27241228	97E10	FBti0226956	97353

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAACAGCTGTTGCACTGCG	GTTCCGTCGAATCTCCTCCAG

Images

Construct Design Sequences

sgRNA sequence	AAAATTAAACGCCCAACGAAAGG
sgRNA2 sequence	TGATGATAAGGAAAGGATAGTGG

 

left_200bp	right_200bp
TGGGGATGGGCGATAACAATCACTGGTTACAGATCACGGTTATCGCAGCTCGATGGGAGCTGTTATGTTATTGAACTGACTATGCTTATCGATAAGAAAACTCAAGCAGGTGTTAAAAGCATCATAGGGTGGCAACACTGTGCCCACTTTTACCGCCAAAATAAATTGCTAGGTTTATTTACGAAAATTAAACGCCCAAC	TAGTGGGAAATAGATCAGTACAATATTATTAGTTAATATAATGAACACACTACTTTGTCATAACTTTTCACAAAATACATTCAACAACAAACAACTAGCATTAGTTATTTTGGGCCAAAGTAGGTATTGTATTATTTGTAAACATATGCCCTGGAGTTTTCTCAGACTCAAGTTTTATATACAACAAGATCAATAATGGC

 

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