CR70541

CRIMIC CR70541

Gene DNApol-alpha60

CG5553

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:20359833..20361585	77B6-77B	FBti0226957	97354

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK?	OK	GAAATATCTTAACAAAGGCAATAGCGTCG	AGAGAAGAACTTTATGGGCAAGAAG

Images

Construct Design Sequences

sgRNA sequence	TTTGGCGCGCAAACTACTGATGG
sgRNA2 sequence	AGAAAAGTTATATTATTCCATGG

left_200bp	right_200bp
TACTACTGGACACTATGTAAACAAAGCTAAGGCCTAAGAAATATCTAACAATGCAACACACAATCAATACATCAGTACCAATTGACTGATAATAACAATTAAAAACGTTTAATTTTGAAATACAAATTGAATAAACCGCAAGCATCGATAATGAGCAGAAAACTATCGATAACAACTGACACAGCTGATATTTGCCATCA	CCATGGTTCGGACTTCACCTCCGGCACAAaTAGCACGTTTTAAGCCTAGGATCAACAGTGTTCTCCGAGTTTCGGCCGTAAGGaGGCCAGCCATTGACATCTACGTAGTAGATGCCCCTGGCTTGGGGATTCATTCGATAGCCCATCGGGGAAACTATGCCAGCAGAACAATCCCGTTTTCTTAGCTCATCCCAAGAAGC