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CRIMIC CR70542

Gene DOR

CG11347

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:4406943..4420648	64B4-64B5	FBti0226896	97197

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTCGCTGTACGTGGC	GCACGTTGCTGCTCCTG

Images

Construct Design Sequences

sgRNA sequence	CGGCTGCAAATGGCGGTTGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GATCACGGCCAGGGTGCTAACGTGCTGATGGAGGAGTCCTGGTACGTAACACCGCCACCATGCTTCACCTCGATTGGGCCAATCAATATGGAAACATCACCATTTGAGAATCTATTGATTGAGCATCCAAGGTTGGTGATCTTTATAGTACACATTACAAGTCTCCAACCCATCCCAGCATCCTACCCGTTCCACCAACA	ACCGCCATTTGCAGCCGAAACGCACGCAAAATACTAGTTATCCATCTGGACTTATTGATCCTTGACTAACTTGCGACTTTTCTATTCTATTTTGCAGCATGTCCGTTTATCATAGTATTAGGTCCACCCAGGAGGGAACCGATAGTTTTGTTAATCTTGATCTCGGGGTGTCGACGGAGGTGCCGCAGCAGCGGGAGGAG

 

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