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CRIMIC CR70544

Gene euc

CG12269

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:18978273..18978809	91E1	FBti0226958	97355

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTAGACCATATAAGCAATTGACTTTCAC	CCAGTTGCCAAAAAGAGAGATAACC

Images

Construct Design Sequences

sgRNA sequence	GTCGAGCAGCGACACTCAGCCGG
sgRNA2 sequence	ATAAATTCTCAAAACTAGCTCGG

 

left_200bp	right_200bp
CCTACTGACCCCAAAGCGCTTGCAGTTTTTTTCTCATGCCCTCAGCCATTCCGCACATCAATATCTTCAATCTTGCCTATATGAAACAAATTGACTTTAATTAAGCATTATGCAAAGCGCCAATCTCTCAGAGCGCTAATCCACAGAGTTTCCCTCAATTGCCTCAATCCAGCACCTAAATTAGTCGAGCAGCGACACTC	GCTCGGTGACAAAAGTAATTATGCATGCATTTCGTTTATTATATGCGACAATTTAACGTTTATTGTTTATTCAATTCACCCCAATAATAAATTATAACAAATGAAAATATTTCTATTTAATTCTAAAGGAAGTCAAGAAAGAAATTAGTAAGAGTTACAAAAATTAAAAAATAAACAATTATCCCACCAAAAAATGTAAT

 

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