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CRIMIC CR70546

Gene Letm1

CG4589

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:24509508..24515320	60D3		98488

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GGGCTTCGAATTTATTTGTTTTCGAG	TGTGGAGAAGGTGCGGGTAATT

Images

Construct Design Sequences

sgRNA sequence	ATCTTGCACCACAAGCCGAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAGAGTTCTTACCGGTTTCGGAGCTCTACTGACGTATCAGTGCTTCGAAAACTTAGCTGCTAATTTAATTATTCGTATTGTTTTCAACGATTTGTTGACTTTGGATCGCGACAACTGGCAATCTTATCTTTCAGCCTATACATATGGCTTAATTTCGATAATATAATTTCTTTATCTTCAACATCTTGCACCACAAGCC	GAATGGTTCTTTTAGGTTATAAAAGGTGGGATTATTCTTATAGACCTTGGACAATTTTTGTATCTACTGGTGACTTATAATGCGACAGATTCAGATTTCGTAGGAAATCACCTGCCAGGGAAGAGGCCCAAAGACCAGCCAGGTACCAGCAAGACCAGACAAAGAGGCACCTGCGGGAAAGTACAGTCATAGTCCAGAGC

 

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