CR70548

CRIMIC CR70548

Gene Pka-R1

CG42341

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:20816118..20836048	77E8-77F1	FBti0226905	97356

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GCTTGTAAAAATGAGTTGTATAGCGAATCATG	GTGGTTGTTCTCTGCAGCATTGTG

Images

Construct Design Sequences

sgRNA sequence	GACTGGTTTGACGTCACTATGGG
sgRNA2 sequence	ATATCGGAGATAGCTTTCCCCGG

left_200bp	right_200bp
ACTAAGCTCAAAATACCATCTGTTCATTCAATATATTGTAACGGACCAGCCTATTTATAGGACACCGATCCTAACGCATTTCAAATGCAGCCATAACTGCCAGGGGCGGCCACTTGCAGTCACAAGTGAGTGACAATTTAATGCAACTTAATCAGCATCTCTGGCCAGCTGGCCATTTTGCTTGACTGGTTTGACGTCAC	CCCCGGATACATCTATATACTTCTCTCAACTTCTTTTAAATTCTCCGGGTCTTTCGAAAAAGAGAGCCCATTTGTATAATCTATGGGACATGCTGCCTCCTCAAAAACGTTTCATTAAATATCCAACAGACATCTGAATGTTTTTAAAAAAAGAAATAAACAAGATGAATATATGTGTAATAACTTAAATCATAAAAGGC