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CRIMIC CR70549

Gene Ppm1

CG12169

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:581337..582810	61C5	FBti0226959	97357

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAACAAGATTTAATGTGCGCAATATGC	ATGCGCACGCATACTACC

Images

Construct Design Sequences

sgRNA sequence	TTCGGAATTCTGTTGGGTTATGG
sgRNA2 sequence	TTTGTAGTGCCAACAGCGTCTGG

 

left_200bp	right_200bp
CTTATTATTACGTGGGAAACGTTTGGCGGATATTAGTAATCCGAGTTGCATGGCAATCCTAATCGTTTCGATTAATCAAGTCGAAGTAGGCTACCGAGTCAGTCGTCTAAATTCAAAAACCCCATTTGGGCTATCAGAGACTGAATATACTATTTTTCTATAGTAGCTAAGAGGCGTGCGTACCTGTAAAAGGACCATAA	GCTGTTGGCACTACAAAATTATCAGTCAACACATCACATTTCCCGGCAGTACAAATATAACACAATACACTACACTTTCCCCCAACTAGCAGGGAAATAAGAACACTTCGTTTTGTATTTTCTCGGGGATCAATGGATCGGATCTCTTGTTTAATTCGAGTTAATGTGCAAATCTATAGTACATTTTATAGCTACGTATA

 

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