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CRIMIC CR70551

Gene CG31793

CG31793

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:19003367..19008234	37B9	FBti0226960	97358

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGACAGTGTAAAAAGGGCTACG	GACATCATCCGCTCGCTG

Images

Construct Design Sequences

sgRNA sequence	ACAACGGAAAACTTTCCATTCGG
sgRNA2 sequence	TATATTGGGCCCAATTGTAATGG

 

left_200bp	right_200bp
TGTATCTAAATCGGCGCGCTTTTGCACACACACACGCACGGCCCTTGCGTCAGCCGGTCGAACAGCTGATTCGGCGCTCAACGGGCATTCGATTTGGCATCGAAGCGCTTGCCATTCGCTACCATTGAACAGAATGATTGGAAAAGCGGGGCGTCGTGGACGGCTCAAGTGATTTGTTGTGTTTTGCGATTCGTCCGAAT	TAATGGAAAGGGAATTTCGTGGTCATAGATTCAGTTTTATAGAATACAAAACGTTTGTACTTAAGGGAGTAAAGCAAAATGACAATGTTTTCTTTTAAATAAAATGCTACTTAAACAACAAGAATCACGTGCCAAATCTTTTGATGATAATCCCAACATTGCTTTCACTGAATTTACCAAAACAATAGTGTTTTTATTTT

 

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