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CRIMIC CR70552

Gene Porin2

CG17137

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10845788..10847044	32B1	FBti0226961	97359

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCGAAACTAGGACACAC	TCCTTCTGAACAAGTTCCGTATCC

Images

Construct Design Sequences

sgRNA sequence	TATAAAACTCGATACTGATCAGG
sgRNA2 sequence	AAGGACGGAAACCATCGGTTCGG

 

left_200bp	right_200bp
GCAGCGTTGGTGGTGGAAGGCTTGGGAATGGTTAGCTTAATTTAAGGATTCAAGCTTGAAAGGAAAAGCTGTATTATAAGATAAGTACCTGATACTGATCCCAAGTTGTTTGTGCTTGAAAAACTTAACTTTTTCGGTTGGGCACAGGACTCCGTGCGTTTTAGTTCACAGGTCTCCAGCGTGTATAAAACTCGATACTG	GTTCGGCGTGGGAATTGCCCTCCAGTGCTAAAATGACTGCCAATCACTTAACGATTTCAATTTCTAAATTTATTTTTAAGTTTATCTTTATTCAAATGACAAATATATTTTGTTCTTCATAATGGCAATACAACAATCTGAGTAATTAATTAAGCAGCTTAAATACAGGCTGTAAAATATGACAAATTAAAAGTAAATAA

 

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