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CRIMIC CR70553

Gene porin

CG6647

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10847227..10850848	32B1	FBti0226967	97360

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
	OK	ACCCCCTCCTCAGTCACTC	CGTTTGTTTGGCAGTGTCTCTC

Images

Construct Design Sequences

sgRNA sequence	GCTAAACGCAATTAATGACCTGG
sgRNA2 sequence	TTCAAGGATTTCACGACTAGCGG

 

left_200bp	right_200bp
AAATTGACATTTAGCCAAAGTGAAAATGGGGAAAAAGCCGTCGAAATTCTTGGGACATGTTGATGCTGTTTTTTGGCGTCTTTTAGTTTTCACTTTTCATTTGCGGCCGATAGAACATTTTGAAATCAAGTCGAGCAAAAAGTAATAATCATTATGTTGCTGATAAGATTAAACTTATCGTTCGCTAAACGCAATTAATG	GTCGTGAAATCCTTGAACGTTGTCTAACATCCCCACACGCCCAACAACAACAACAACAACAATAACAGCAGCCAGACAAGTGCAGAAACAACAACAAGATCAAACAAAATAAAAGATACGATATTCTTCAAAAACAAACAACACACAGTCAAATATAAAGAGAATCTGCACATCTTAACGATTCAATTGGATTGGATTAG

 

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