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CRIMIC CR70556

Gene Vps11

CG32350

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:23147656..23150499	80D3	FBti0226962	97361

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTCAGAAATCCGAGCTTCG	CGTCACGAAAAAAGTGGCTGC

Images

Construct Design Sequences

sgRNA sequence	ATATGAAACTATTGACAGAATGG
sgRNA2 sequence	ACATAAATCGATACATTATATGG

 

left_200bp	right_200bp
TATTATATATCCACATCTAAGATTAAAATCATAACAATTGGATGTGCTTAGTGTAATTTGCTTATAAAAACTACTTTATATTTTCATATTGCACTGTTTCATTTTGTTGGCCAACCCGTAACGTCACACATGTTCCACTATAAGAATAAATATACACATTAATAATAGTATCATCGCATAATTATATGAAACTATTGACA	ATATGGCACTGCCAAGCCACCAATTTTCTATTTTAAATGTGAGGTCTTTCTTAGAAATTAAAAAAAAAAACAATGTAATAGCAAAACTAAAAACAAAAATAATAAATTTTCTGGCAAGCATTTTGGCAAATCCAGTTTTTTACCTGTCCAAGAAATGTATTTTCTTTGATATCCCACTGTTACCACTGTTAGATGCGAAA

 

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