CR70562

CRIMIC CR70562

Gene CG31915

CG31915

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:5528123..5530682	25E5-25E5		98548

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGTATGAAATGTTCTGCCTTGAAAG	CCACTGATCTACAGTTTTGAGGTGC

Images

Construct Design Sequences

sgRNA sequence	ATGCGCCGGAGAATCAGATTCGG
sgRNA2 sequence	ATGCGCCGGAGAATCAGATTCGG

left_200bp	right_200bp
TTATTAATCAAATGAAAGGATCTTTAAGAAACTATGGAGCTAAACTATGGATTTCCGACTGAAATTCATTTAAATCCATTAAAATCGATAACAAACGATAACAGCACAGTTTGAGAGGCGCCGCAGCTGATGCAGTTTGAGCGGCACTCCATCTGGAGCTGGAAAAGAAGGAGGCAGTTTATTAATAGATAACGCCGAAT	AACTGCCAAATGTGCCTTTAATCCAAATCCACCAAAAATATGAAACAACGATTGCTAGTTTAAGTTAATGTTTTGATTAGCCGACCTAATATTTTGTGCAATTCTTTCGAACTTTTAAACTCGCCAAAAATGTTCCTAAATGAAAAAAGTTTGTTATCTAAAAGATAACACAAGTAGTAGTAATCTATGTTATAAAGTAA