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CRIMIC CR70564

Gene zfh2

CG1449

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	4:524125..524129	102C4		98491

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGATATTTGAACTTGTGACTGGATCTG	CGGAGACTAAAAGTGGTGTGGAC

Images

Construct Design Sequences

sgRNA sequence	AAACTAGTGCACCTGTTGGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACATGCTTACAGGCTGTTCATTAAAACGCAAATGCCGATTGGGTTGATTATAAAAAGCCCTTCGGTTAGTTGCATCCCACTAATTGCTTCACACTTGAATAATCACGTTTGCTTCCAAATCCCAACCGGCATTGACAAGAAGCACCAATGAAAATATTCTTTGGTGAATTTGTAGAGAGGAGAGTGTTAAATGCCCTACC	AACAGGTGCACTAGTTTTTTTCATTCTTACAATTTAATGTTGTTGGAAATTTGTATCACGCGGAACATTTAGGTTGCTTCTTTATTAAAAGGGTAAATATTAgATTTACAAATGCGAACAGAAAGCAAACAAGATTATAAGAATATACCATCATAAATTGTTGAATGGAGCAGAGTACATTGTATATTACGGATCTATTC

 

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