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CRIMIC CR70565

Gene Gyf

CG11148

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	4:853557..867025			98492

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTTCAAACTATTCAAATATGGGCTTGC	CCAGATCCTTTATTGTTTCCAGTCC

Images

Construct Design Sequences

sgRNA sequence	TTGCAATAGCTCGACTATAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTGGATACTATAATAAATTGTATATATAATGTATTTATAAAATTTTTGTCGTGCTTAGTGATTAAGAGTTAGTGTTCTGCCGCTATGTGGATCAGGTGTACAATCACTGACATATTTGCTTTCCTACTTGTATATCTGAATATCGCTTGCATTTCTAGTATCTAACTAAATGGGTAACAGTTTTGCAATAGCTCGACTA	TAGCGGTTTTCTGTTTAGTAAGTTTGCTATATTTGCTAAGAAGATGAACCAAGTTAAAATTTAAATAGTCCAAGTTATTGAACAACAGTATAGAACTTAAAAAAAATTTTTTTTTAGGAAGATCTACAAGCTGGCGTGATGAAGATCCTAGCGTCGACAATGCAGCTAGTTTACAACGCTCAATTTCAACAGTTGCGACT

 

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