CR70567

CRIMIC CR70567

Gene CG3491

CG3491

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:14924590..14930565	35B7-35B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTCATAAATTATAAGCACATTCTTTTGGG	ATGAATCACATACGCACGCAG

Images

Construct Design Sequences

sgRNA sequence	TTGCTTTCGCCAGTACAAGTCGG
sgRNA2 sequence	CTGTGCGCTTCGCCGTACACTGG

left_200bp	right_200bp
TACTTGTATTAAAATTTGAAAATAAATACTAAGCAACTCTTTTATAACCTAAAAAAAATAAATTTGTTGCCAGCATTTAGCCAGCTGGCATTGAGCTGCCCTCTTAATCAACTTAATAGGCTGTGCGAAATTAGGAAACACTATAGTCGCACGAAATAGCAAAATTTTcCTCAGTCGATGAGATTGCTTTCGCCAGTACA	TACGGCGAAGCGCACAGCACTTCTATTGCCACTTTAAAAATATCAACCGAAATTGTAGCATTCAATGCAATACATGTGGCGTAAGCTTTCGCTTTTTCAAAATGCCAAGCACGCTCCACCAGCTCCACCCAGGAGGAGCAGCACGCCCATGTTCCCGGCAATAAACAATGGCCACAGAAGCACATACGGGAAGACACTTA