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CRIMIC CR70568

Gene Slu7

CG1420

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:29037058..29039071	98F5-98F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGCTTTAAAACTGTTTCGCG	CAGCAACGGAACTCTGGC

Images

Construct Design Sequences

sgRNA sequence	ATTATCTAGTAAATAAAATCAGG
sgRNA2 sequence	GCCTACATAAATTGCAGCATGGG

 

left_200bp	right_200bp
AAAATGTTCACTTCACTCCAATTCTTATTGGAAAAACGTTAGCGATGTGCCAAGGTGGGCGATACATATCGTGGGGGTGCTACAGATTGTCCAACACTTTCGCTGTTAAGCCAAGTGCTTCTCTGCTTGCCAACACTTTTCGATAACCGATAACATAAAAGAGAAATAAATATTACAACTAATATTATCTAGTAAATAAA	CTGCAATTTATGTAGGCAATAAAGTACAACTGATTCTCTTATATAAAAACTTTCTAATCTTAACAAGTAAAACATTAGGGGCTTACACAAACGTCATTTTGAATATTAAACGATCGCATACTGCTCGTACAGCAACTCCCTGATCTTGTAGTACTCCTCGGATAAGCATCCCTCCATGGCTACCTTGCCGGCATCCACTC

 

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