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CRIMIC CR70570

Gene CG5446

CG5446

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:12426631..12427709	33E4		98550

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGGGGCAGTGTTGAAAAACG	CTAAACGGCAATAATTCAAAATTATTGGC

Images

Construct Design Sequences

sgRNA sequence	TCAAGCATAGGGGCGGACTTTGG
sgRNA2 sequence	TGTATGTATCTAATGGTATTGGG

 

left_200bp	right_200bp
TACAATTTGGGTATATTCTCTGTAATAATACATCCCATAAAATGATGTTGAATAAAAATGGTTCTTAATATTTATTTGAATTATTTAAAGATTAAAATAAATTAAAATAATAAATTGACATTAAATATGACTGGAAAATAAAACTTGAAGCTAGCTACTTTGCCACCACCAGGATTATCGAAAGGACTATCGAACCAAAG	ACCATTAGATACATACATCGAACACCTGGCACCCAGTCACCCGTATCAAGTTTACAATTCCAGGTTCTCACATTCATTTAATCTCCTCCGCACCCAGCTGCTCCCCAAGAAATGAAGAAAAGAACACATTTGCTCATCCATTTATTGCATAGTTTTAAGTTTCGCCCGGTCGGGGCACATCATAATTTAAGTAAAGTAAT

 

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