CR70574

CRIMIC CR70574

Gene Hel25E_newer

CG7269

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2L:5539326..5542314	25E6-25E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CAGAAATGGCCGACAATGACGATC	CGAGTTCCCAATTACCATTTCACC

Images

Construct Design Sequences

sgRNA sequence	CACTCGTGCTGAACTGCAAGCGG
sgRNA2 sequence	TGAGGGACGCTAGAGCTCGAAGG

left_200bp	right_200bp
TCGAAAACCAGGAGGCCCCCAAGAAGGATGTCAAGGGCACCTATGTGTCCATTCACAGTTCCGGCTTCCGCGATTTCCTCCTGAAACCGGAGATCCTGCGCGCCATCGTAGACTGCGGCTTCGAGCATCCCTCGGAGGGTGAGTACTTGCACTCCACCTGGAGTGACGAAAGGTACAGTCGCTAACGATGCTCTCCGCTT	CGAAGGTGCATGTTAATCGGAACAAGACTATGCACTTCGAATAGATACAAACCTAAAACCTTTTTTAAAACTCTACTTTATAACTAAATAAAAACTATTCAGACAATGTGTTGACAAACAAAAACGGAATTATAAGTAATGTATTTATTTTGGGAATGGATTCTAAAACCCACTTGTTTCACATTTCCGTTTAAACTATT