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CRIMIC CR70583

Gene mRpS5

CG40049

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:24537236..24543534	80F-80F		98496

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTCTCTTCAATCAAGCTTGTAGCAG	GTCCATGGCCATATCGACTCG

Images

Construct Design Sequences

sgRNA sequence	GATTTACAGTGAAATACAGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCACATGAACCCATTTTACCAGGTTACATTGGTTTACGTAATACCAGCTTCTTTAACAAATGTAAGTAAAATGTTTTAATTCAATAAAAGAGCAGAAATGTTTCTATCTGCCATTGGATAAAAATGGTTCTAGGTAGTAGTAATGGTGCAAATAAAATGAAACAAGGAATTCTTTTTCTGGCTGATTTACAGTGAAATAC	AGTGGGTCAGAAATTATTTCCACGAGGTGTAATTTGAATCTTTCAAAGCCTTTAAAAGAAAAATGTCAGAATCATTATGTATTTTTTATTTAAACAACAATGATTTATTATTTTCGTGTATTTTTACAAATATTATTTTTTCCTCATATTTAAAGGACTTTGCAGTAATAATGACCCTTCGTATTTAGAAGTACCGGGTC

 

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