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CRIMIC CR70584

Gene eIF4H1

CG4429

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:16448665..16454972	14C6-14C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGTGCGTTTTGCCCCAAG	GCAAATTGCCGACAAATGCG

Images

Construct Design Sequences

sgRNA sequence	TTGGTTGCGCAAATCTATAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCACAAACCACCACCACCCCCCCCCTCACACCACAGTCCCTCAAAAGGGAAGAAGAAGAAGAGACAGACACACCCCCATTAATTGGATTCGATGTCAAGGTTCCCAGGCCACGGAGTAATCCTCCACACAAAGGGGCACAGCAAATACCAGGAGCCAGAATCGAGCACACCATGCAGTTGCTTTGGTTGCGCAAATCTA	TAGAGGGGGATCTGTAATCCAACTTTAGAAGCTAACATACTACATGCAGAATAGGTTTTTCATGGGTGCATCCCATTTCCAAGATTCATTGCCTTTAAATTTATTAGTGTACTTACTCTACTAACCATTCATTTTTATTTCTCGATATTTTTCAGAAGCGGATTCGGTGGGGATCGGGCATCCAAGCAGTTGCCCACGGA

 

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