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CRIMIC CR70585

Gene spartin

CG12001

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:4654800..4657985	82C5-82D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAATCGCCGACCTCCCAAC	CCAGCTATCCATCCTGTCATTCC

Images

Construct Design Sequences

sgRNA sequence	TTTATCGTTACATGCTCATTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTGATTTCATTGCTAGTAACCTGGTACGGGGCGCCGAGAAGACAGGAGGCTTTATGCTTAGGAGTACTCCGTATATCATTTCCAAGATGACGCCAGCCTCGATGGATGCTCAGGTGCCCAGCTCCGTCCAAACTTCAGGTGAGTTAGTTCCAAATAGGGAAGAGCTGTATTTAGGGCGGAAGTATGGTAGCTTCCGAAT	GAGCATGTAACGATAAAATTAGAAGATAAGATTCGAAGTCGAAGATGTGATCGTCACTACACTTCCACCTCGATCGGAGTTTGTGCTTGTGTTTGATAATCATGTTAATCAATGGTTTGGAAATGTGCAGATTACATATAAGATAGATCTAATGTATCTTATGTTGTAACCTTTTGTTCATTATAGTTGAGGTGGCCCAA

 

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