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CRIMIC CR70586

Gene Cul2

CG1512

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	2L:21658735..21663698	39E3-39E6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCACCAAAGTGGAGCG	AAGCAGCCTGCGTTACACTG

Images

Construct Design Sequences

sgRNA sequence	ATGAATGTACTAGTACCTAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAGGTACGTCTTGGCAACGGTCGGAGTATTAAACAGCCTGGAGAGAGATATTGTGATAGGTCACTAGAGACTACAAAGTTACCCAACTCAGAAGAAACACAAATAACACTAACTAACTTTTAAATTAATCCAACATAAGTAATGGTTTCAATCACATTATTTTGCATTTGAGTTGGTAAACTATGAATGTACTAGTACC	TAGCGGTTCAACTATATATTTTATTCAACAGCAAAATTCACAAATATAGCTGCTAGCGAGGCGAAAATGATTTGTTAAATGTCGTAGAATCTTGATAAATGCAATCATAGTTTTAATAATAAATGCCTGTTTTCTAGCTTGAATTTAAATATTTTGACCGCTTAAAGGTTAAACTAAGTTCAAGTAAATATTAAATAGAT

 

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