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CRIMIC CR70587

Gene Coq2

CG9613

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:8314784..8316543	84F11-84F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACTACATTCCGCATATGTTCTTCTG	GTAAGTTCTTACGTCAAGGCGG

Images

Construct Design Sequences

sgRNA sequence	TCGTTGAAGGTGCTAGTGCAAGG
sgRNA2 sequence	CACTACAATCTACGCTCGTCTGG

 

left_200bp	right_200bp
CCAAATGCAGCAGTGCCACCGGATCATATTGTTTTTTCATCTATCCGGCAACTCTTTGTCTGCCTTTACCCGGATGGCAGCCCTTTGACATTCCCAACCAAAGTTCCATTAAACATCTTGCCATTTTTTGACTGTGAGTTTACGAACAAATACAACTTAACATCGTGTGACTTAATGCTTTAATCGTTGAAGGTGCTAGT	GAGCGTAGATTGTAGTGTTAAATTATGTTTATAGCGCGCTTTTTGATTCGCCAAAACCAAAGTCTCTAGGTATCTGATTAGATAAAATCTTGTAAATAGGCTTATGACCATTTTTAGCTCATTTGCAATAGGTTAAGTCTTAAAGAATATTACTAAGTGCACACCTCACTTAAACATCAATTAATTCTTAATTGTAAGTG

 

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