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CRIMIC CR70589

Gene GCC88

CG10703

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:11228158..11230600	86D8-86D8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGATGAAGGACGAGGAGGG	GCGGAAATGTCCACTGCTG

Images

Construct Design Sequences

sgRNA sequence	CCTGAGCAAACATGACAAGTTGG
sgRNA2 sequence	CTATTTAGGATCTGTTTGTAGGG

 

left_200bp	right_200bp
TTTACTTTAATTGGACTAATATTAATGTTTGAAAATATCTCAATTTAGCAACTATGCAGCTGATTTATAATACATTTACATTTAAAATAGTTTTTAATTTATGTAAATTTGGTTATTAATTATTTTTTGAAAAACCGCAAAAATAAAAAAAAGTTTAAATCAGAATGATGGCAATTTTTAGTGCCTGAGCAAACATGACA	AAACAGATCCTAAATAGATAACTAACTAAATCCGACGACTGGGTATTTGTCTAGGTGGGCAGCTAGCACGCCGGGAAACATGGCCAGGAACTTAAAGCGAGTGCCCATCTGGGCTGGATCCGTGAGCATCTCGTAGCCGGAGCGAATGATCTCCTGGTTTTCGGGCAGCGCATGTGCCAACAGTTGCTCCAGTCGAGCCT

 

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