CR70590

CRIMIC CR70590

Gene CG31800

CG31800

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:19033657..19034576	37B10-37B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCACTTATGGTCAATTTCGC	TGTTGCACTAAAAACCAAAACATACATCG

Images

Construct Design Sequences

sgRNA sequence	TACTTATTTGTATTCATGTTCGG
sgRNA2 sequence	TATCAGTAACATCGAAGTTTGGG

left_200bp	right_200bp
CAATTTAGTGTGCTTCTTCTTTCTATCTAGAGACGGCCGTGCAATATGACATCTATTCGATAGTGATCGATAACACCTAGGGAAAACGATGACTGGAGGAGATATACCAGCTATAAAGAACACATGTTATTATTTTATTTTTGGCAGTCCATAAATTTACATAGTTGTCAATCGCGGAGTGATTACTTATTTGTATTCAT	CTTCGATGTTACTGATATTAAAATTATAAAAGCTCTAGGGTAAAATGTATTAATTTTAATTTAAATATTAACATTTATATTTTTATAAACATACATATTTAATTCCTGTTTAATTTTGTAAACATGATAAATAATAAATAACGATATTTTCTATCGATAGAGAAAGTGCATCTCAATGGATTTCGATAGCTGTCGTGCAG