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CRIMIC CR70591

Gene IKKepsilon

CG2615

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:20676045..20678979	38C7-38E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGGCAGCTATCGAAGTAGCAG	CAAGAGTCATCATCATCAGTTTCGTC

Images

Construct Design Sequences

sgRNA sequence	TTTGTTTGTCACAATCGAGAAGG
sgRNA2 sequence	CATATATTCCTATTGAAACTTGG

 

left_200bp	right_200bp
TTAACAAAGAACATGAAACGGGTTATAATATTTCCCTAGCAGATATGTTTAATACAATTGTGTGTGTACGTAATAAAACAGACAATCTAAATAGAATAATTATATATTTTATTGATAGTTCCAAAATAAACATGTTGTTTTGCAGTGTTATCGTATTATCGATAGACCTTCCTTACCAGTGTTTTTGTTTGTCACAATCG	ACTTGGCTGAATATCGTGAAAGAGTAAGATTTCTGTAGGTCATTAAATTATTTATTTTATCATGCTGAAATAAAATTAAATTTCCGATTATAGTTATTTAGTTATTGTTATTTAACTGTCTATACATGAAAAAGCGGACTAGACTGCATGGCACACACAATTTAAAAATGGTAGAATGCCCTATGGCTAGAATGCACTGT

 

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