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CRIMIC CR70593

Gene CG5731

CG5731

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:10207085..10209744	31B1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAGATTTATGCGGGAATACAGC	GCATCGTCGCAAAATACCATG

Images

Construct Design Sequences

sgRNA sequence	ATAATCAACTGAAATCGGATTGG
sgRNA2 sequence	TGTCAGTCGCTAACGCAGAAAGG

 

left_200bp	right_200bp
TCCAAACGCACTGCAACCTGTGGAGGAACTATGACGACATCCAGGACTCGTGGGCATCCGTGGAGAATATAATCGATTATTATGGCAACAACCAGGACGTAATAGCGCCCAACGCAGGTCCTGGTCATTGGAACGATCCAGATATGGTGGGTATTAATAGGTTTTACCTTTGAATAATATATTTATATCTTTTCCCAATC	TGCGTTAGCGACTGACAATTTAATAAAATATTCTCTAGTGTATAGGTTCAACATTAAATACTCACACAATTTATAATGAATATTATTTCTAAGCCTCTACAAGCCAATGTTTAGGTTTACATATATATCTAATAAAAGTAATCAAATTAAATGAGCGTATTTTATCTATTATACTTATTCAAATTAAACATTTTTAACGA

 

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