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CRIMIC CR70594

Gene Ntf-2r

CG10174

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:18454649..18455419	36F5-36F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGTTTGCCCGTCCTCAG	GGTGCATTTACAGCGCACTTTTGG

Images

Construct Design Sequences

sgRNA sequence	TCGGTTAAGCTGACAGGCTTAGG
sgRNA2 sequence	TATTAGGCGCTGGGCGTCTTTGG

 

left_200bp	right_200bp
CGGAATCGTTCGGTTTTAATTAATAAATGAGCTTATTATTAAAATGACGTTCGCACGAGTGCGGCTGAAACCGATGTCTCTTCGCGCCTATCGATGTTTACCTGCGACAATCGATTGTTTTCGGTGGGACCAAAACGTTCAAATCAAATGTATCAGCTTAGCGGTGACACAAACGAGAGGATATTTGAACAGTGCCTAAG	ACGCCCAGCGCCTAATAGTGATAACATGATAACAACAGCACGGCAGTGGAACTCAGAAAAAAGCAAAACAAAGCCAGCCAACGGTCTCAAGATCGTCAGCGAATACAAAAGTTAATACAAGACAAACAAAAAAATAATTAAAAAAAGCTTAGGACTTTTATTTTGAAAACTTTTTCGAGAAATAAAATAGAAACAAATCT

 

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