CR70596

CRIMIC CR70596

Gene elgi

CG17033

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:15956550..15958433	72B2-72C1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGTATTTAATTTGTTAAGCTGCCTG	GAGAATGAAGTGAACGATAACAAAGGG

Images

Construct Design Sequences

sgRNA sequence	ATCTTAGGTCCTCAGCACGCTGG
sgRNA2 sequence	TAATGCTTACTCTTACGTTAAGG

left_200bp	right_200bp
AATATCTCAATATTTTCAAGCTAAACAGTTATTTTAAGAAGCCATTTCTCGCGGATTACAGCTGAAGTGCCATCCTAGCTTTCCAACACTACTCAGTCACACTACACAACACTGCACTGACTGAACCGAAAAAAAAACACACAGATTTTGCAATCCGCAGAAAAGAATTATTAATAGTATTTTTATGCGGATGGCCAGCG	CGTAAGAGTAAGCATTAAAAACAATATTGTAAGGAAAAACTCGCAAAACAAACCATTCAAGCGCCTCATGACGACTTTCAAGTTGTACAAAAAATATATATTACTTTCGGTTCATTGTGTTTTATCCATTTAGTTAGTACGCAAATTCGTATACATCATCAAGTCGTTCGCATATACCCAATATTACATGTTTGATTAAG