CR70600

CRIMIC CR70600

Gene Nrd1

CG2025

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:11864651..11868893	10F2-10F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ACCACACTGAATCACTAATCAATTAGC	TGTACATATAGTATGAATAAAACGATAGATGCGG

Images

Construct Design Sequences

sgRNA sequence	TCGAATGAGAATGAGACTTATGG
sgRNA2 sequence	GATATCCTCCTGACCAAGTCGGG

left_200bp	right_200bp
CAAGCCATTTGACATTTGCGGCGTTATCGATAAGGCGACACCCAAGCACCTGGTCCATCACTAAGCCCACGTCTTTCGATTAATAGTTTGTATCGTTAACATATAAAATAGTTGCCTTTGCGTTTGCTATTTGACAGCCTGTGATTTTTTCCCCGTGGATTTTCCATAGCTCCGATCCCAGTTGATACGGATTGCCATAA	GTCGGGAATTTGCTCTTATGTTGTAATACGCTCATTCATTATAAATTAAAAACACAGCAAATGACGAATGCATTTTGAAGCATGGAGACACCGTATATGTTCAATTGCACTGGACATTCGTACTGATTTAATACAAATACGCGAAAAAAAGAATTCAAAATTTGAAGTGATCAATTTGCCTAATTAGAAAACAAACATCC