CR70601

CRIMIC CR70601

Gene CG10417

CG10417

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:5360331..5363104	41E5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGTATAGTTTTCCAAAAGTCCTACCAAG	GTCTTTGCAACTAAAGGTATTCGG

Images

Construct Design Sequences

sgRNA sequence	AGGTTACAAGTTGTACAATATGG
sgRNA2 sequence	AATTATAGTAACTTCCTTAATGG

left_200bp	right_200bp
CGTTTTAAATAAATATATATAATAAAATTATTTTTCTAACTAAAAAACATTACAGTAAATGTTTGCAAGTACAGCCTATACGTAGAATTTTATGCACAAAACCTTATCTTGGTAAGCCATCGATTTTTTTTATTTTAAAGGATAGTGCAAATTGAGTTTTTAACCTATTATAATATCCTTGAAAGGTTACAAGTTGTACA	AGGAAGTTACTATAATTGTTTCTTCATCGTAATTTACTTGACATTCGGAAGTGCACTCATCTTAATTCACATTTAATCGTAACATGCATTTTAATTGTAGACATTTAAGCTTGTAGATCGAAGTGCGAAAAACTGTGCCACTTTTCAAAATGATATTGATGACAGGTTTATGCAAACAAATATTCTTTAATAAGAACATA