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CRIMIC CR70606

Gene AnxB9

CG5730

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:21065241..21070917	93B9-93B10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCGATTAGCGCCGTTAATG	GTGTGCACTGCAAATCGAATATAGG

Images

Construct Design Sequences

sgRNA sequence	GTTGACAAACAAGTACCGATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCAAATTAGATTCAACAGATAGATGAGAATATCTTAATATTATTTTTTCTAAAACGTGTGTTGTTGTCAAGTAAGCAAGATTTTTCTCGATGCAATCTATAAATTATTACAACGACCAGATGCTACGAAATTATCTATAATTGGGCTATTAAATTATCATCAGAGGTGTATACTAGACAATCGTTGACAAACAAGTACC	GATTGGTGGGAGAAGAGAGTGATAAGAGGGTTTCAGTTATGAGTTCCTTAGATAAGAGTCACAACGAAAAAAAAAGTCAATTTAGAAAGCTAACTTTATTGCAGAGGAATTCATGCAATACTGAGATAATACATGTAGGGAATAACCACATATGTATTTGTATTTAGAACAAGTGCCACTGAGTAGTGTTGAGTCATTTC

 

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