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CRIMIC CR70610

Gene DCTN1-p150

CG9206

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:13929387..13934656	70C5-70C6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACCTTATTGGCTTGCATTAG	TCGCGCAGATCAAGCTCC

Images

Construct Design Sequences

sgRNA sequence	ATTTAAAAACGAAACTATACAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAGATCTCTAGGCACTTTTAACCGATATCTTTAGTTTACATGTGTAATATATTTTGCATAAGGTCAATCTAAAGATAAACTTTTTCATTGCATTTCTTTTTGCCGGCCTGCCTTTAAACCTAATAATTATCGCGGTTCAAGGAGGTAAATTGTGTAATTTCAACTTTTCCCCTGCCAAAATTTCAATCATATTCCTGTA	TAGTTTCGTTTTTAAATTAAATTCAAAAACGAAGAAACTAAATATATTTATTTTGTCCCAGGAACAAGTCGATGCTGCACTTGGTGCCGAGGAAATGGTGGAGCAGCTGGCTGAAAAGAAAATGGAATTGGAAGACAAAGTAAAACTGCTCGAGGAGGAAATTGCCCAATTGGAGGCCTTGGAGGAAGTGCACGAACAGC

 

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