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CRIMIC CR70612

Gene Elp3

CG15433

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:4444732..4446765	24F2-24F2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTTCCTTTAGCAACTGCTCCTG	GAGTGGATCGATTTATGTTCATGCTG

Images

Construct Design Sequences

sgRNA sequence	GATATTCATCAGCAAAATGAAGG
sgRNA2 sequence	ATAGCGTTAAATGACTGTCTTGG

 

left_200bp	right_200bp
CAGAACTCCCGAGAAACTGGTGCTAAAACAAATAAATAATCGACAAACGATAGACCATCTAAAAGTGTTACCAAGTGGTGTTAGCTTCAAAACCTGTCAAACAGTGTTGTGGTTATCGGACTATTGGTCGGTCGATAACCGAATCAAAACAAGTGCTGCTAACTTCACACGTGCAGTTTATGTGATATTCATCAGCAAAA	TCTTGGTGGTATGTTGGAGGATTAAATATTTGTATTTTATCACGCTAGGAGCAGTAAGATTTCGCTACTTAAAACTACTCTCTTAAATATATACATTAATATATAGAATGAATCGATTTATTGGCTAAAACTCACAGGGTCCTTTAAAGTATCAATGATACCACATATTTTTTGGCTTTAACATCTCACAAGAACAACTA

 

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